|
|
Welcome to UCLA Human Salivary Proteome Project
Director: Dr. David Wong
Co-Director: Dr. Joseph Loo
|
T
he “Human Salivary Proteome” proposal is a multi-institutional multidisciplinary research consortium
responsive to the NIDCR RFA DE-04-007 aiming to generate a complete catalogue of all salivary secretory
proteins using state of the art, sensitive and high-throughput proteomic technologies. While there have been
substantive inroads into the salivary proteome using traditional biochemical approaches, there is an urgent
need to comprehensively catalogue the entire “dictionary” of all salivary secretory proteins and their
complexes employing state of the art mass spectrometry and accompanying emerging technologies.
This proposal is the collective efforts of investigators from UCLA and USC, in conjunction with the
consultant expertise, proposes 3 broad Specific Aims to address the multifaceted nature of this RFA. Each of
these Aims are the efforts of investigators with significant background and expertise to address the clinical
resources, proteomic competencies and informatics capabilities to address the comprehensive nature of this
proposal. Aim 1 (Clinical Core) is to identify and develop resources to support and implement clinical
acquisition of saliva for the proteomic program. A cohort of human salivary donors with distinct parotid,
submandibular and sublinqual fluids who will provide the necessary salivary samples will be identified. Aim 2
(Proteomic Core) proposes multifaceted proteomic approaches to decipher the complete salivary proteome
and their associated complexes from the three major normal adult salivary glands. Emergent technologies,
bottom up and top down proteomics, and others, will be used to populate the complete catalogue of salivary
proteins. Functional proteomic approaches will be used to unravel salivary protein complexes and
understand the function of the proteins. Aim 3 is the biocomputational and bioinformatics plan to catalogue the
salivary proteome, annotate the salivary proteome and effect the fully Salivary Proteome Knowledge Base
(SPKB).
We are excited to present our collaborative consortium. Our consortium is a multidisciplinary team of
biologists, chemists, proteomics leaderships, engineers, computer scientists collectively focusing on the goal
to create the periodic table of salivary secretory proteins.
|
|
